Journal: Journal of Nanobiotechnology
Article Title: Fisetin carbon dots alleviate periodontitis by enhancing mitophagy through regulation of sirtuin 3 SUMOylation
doi: 10.1186/s12951-025-03907-9
Figure Lengend Snippet: FIS-CDs alleviated H 2 O 2 -induced oxidative stress, restored mitochondrial function, and promoted mitophagy. ( A ) Assessment of intracellular ROS accumulation via DCFH-DA based fluorescence staining. ( B ) Visualization of cell morphology by staining F-actin with phalloidin. ( C ) Measurement of MDA content in hPDLCs. ( D ) Assessment of T-AOC in hPDLCs. ( E ) Fluorescence images showing the co-localization of FIS-CDs with MitoTracker Green. ( F ) Quantitative analysis of the co-localization between FIS-CDs and mitochondria. ( G ) MitoSOX staining for evaluation of mtROS production. ( H ) Quantitative analysis of MitoSOX signal intensity. (I ) JC-1 staining for assessment of MMP. ( J ) Quantitative analysis of JC-1 signal intensity. ( K–O ) Western blot analysis of mitophagy proteins: ( L ) PINK1, ( M ) Parkin, ( N ) p62, and ( O ) LC3B. ( P ) Quantification of DCFH-DA fluorescence intensity. ( Q ) Quantification of intracellular ATP levels. The data are presented as mean ± SD ( n = 3). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant
Article Snippet: The intracellular concentration of adenosine triphosphate (ATP) was quantified utilizing a commercially available ATP detection kit (MCE, USA).
Techniques: Fluorescence, Staining, Western Blot